Monday, November 4, 2013

catalase post lab

1.        What was the purpose of performing the splint test-To see if oxygen was present in the test tubes
2.        How did you know which substances had active catalase present-There would be bubbles and flame
3.        What effect did cooking of the potato and liver have on the results of this test-It cause there to be no bubbles or flame present in the test tube
4.    What implications can be made to the real world from this experiment?-Then the bubbles in your body would expand.
5.    Have you ever used hydrogen peroxide on a cut/scrape/wound?  It is used as an antiseptic (It would foam as it encounters the wound area).  Based on the experiment you just tested, what do you think is happening when hydrogen peroxide bubbles on contact with cuts and wounds?-I personally ave not but i bet the the cut or wound would foam.                                                                                                              6. What did you observe in the fruit juice test tube? Explain what may have caused these results.-There were no bubbles or fire probably because there is not very much water in fruit juice.
7. Explain how your liver gets rid of toxins. Is catalase the only enzyme at work? What else needs to be present for your body to fully get rid of.-I am pretty sure that catalase is the only enzyme at work.Your liver uses water and oxygen in your body to destroy the toxins. 

8. What is yeast? Why did your yeast tube get the reaction that it did if yeast is not part of a liver cell?-east is a part of bread that uses oxygen.It got the reaction it did because of the water and oxygen used to make bread
9. Design an experiment you could do on your own that would test for the presence of lactase in food. Lactase is the enzyme that breaks down lactose (in milk).-You would use 3 vials of different vials.In one there would be normal milk,in another there would be skin milk, and in the last there would be chocolate would poor 5ml in to each and you would measure the reaction.

Thursday, October 31, 2013

1. What is an enzyme?
Chemicals that aren't consumed in a reaction but speed up a reaction. 
2. What elements make up the type of macromolecule that categorize enzymes?
3. Explain how the above reaction might take place or look inside of the liver cell.
hydrogen peroxide is broken down by catalase and turned into 2 H2O and O2
4. Your liver has the specific function of helping to break down toxins in your body. Why would your liver need many different types of enzymes instead of just many of a single type of enzyme?
There are many different types of toxins so your liver need many different types of enzyme to fight them off. 
5. What effects do catalysts have on an organism’s metabolism?
 they cause organisms to grow and reproduce
6. All enzymes end in the suffix “ase.”
7. Where is the enzyme catalase found?
It is found in nearly all organisms exposed to oxygen 

Now, begin to set up your lab report by pre-writing the Title, purpose, materials, and Procedure.
Title: Catalase lab
Purpose: (read though the materials I gave you and the procedure, what do you think the purpose of the lab is?)
Materials: Make a numbered or bulleted list of materials from reading the procedure
Procedure: (rewrite this IN YOUR OWN WORDS!! INCLUDE ALL STEPS!)

Safety notes:
  • You must wear safety goggles while performing this lab and until everyone in the class has finished and put away their materials.
  • Hydrogen peroxide is a powerful bleach - wash it off with plenty of water if you get it on your skin.
  • Make sure to wash your hands carefully after handling the liver.
  • When you have finished, discard potato and liver in the trash (not the sink!) and wash the test tubes out with detergent.

1.Predict what you think will happen

Label the test tubes with letters A-F

3. add one of the following substances to each tube
a) 1 piece of chopped raw potato
b) 1 piece of chopped boiled potato
c) 1 pipet full of fruit juice
d) a small piece of raw liver
e) a small piece of cooked liver
f)  1 pipet full of yeast suspension

4. Measure 10 ml of hydrogen peroxide into one of the 6 test tubes using a graduated cylinder and record any qualitative observations in the data table.

5. Light a wooden splint, and then blow it out so that it is glowing. Gently push the glowing splint down through the bubbles in your tube.

6.    Record your observations and complete the table.

7.    Repeat steps 4 – 6 for the other 5 tubes. Remember to record your data!

8.    Answer the questions at the end of the lab.

Tuesday, October 29, 2013

If I could meet a famous scientist I would want to meet Charles DArwin because his theory of evolution is awesome.